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15
2024
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11
Research Assistant | Selected Molecular Diagnostic Premix
Product Advantages 1. High Sensitivity: Detection down to 25 copies. 2. Multiplex Target Amplification: Multiplex target amplification is highly specific.
Author:
Research Assistant | Selected Molecular Diagnostic Premix
qPCR Series
RT-qPCR Series
Enzyme Series
Product Category and Performance Introduction
01 High-Performance qPCR Premix
Product Advantages
1. High Sensitivity: Detection down to 25 copies.
2. Multiplex Target Amplification: Strong specificity for multiplex target amplification.
3. Convenient Operation: One-tube all-premixed reagent, only requiring the addition of primers, probes, and template.
4. Products are available in lyophilized liquid, lyophilized powder, and lyophilized microsphere forms.
Product Performance Test Data
★Repeatability
Using whole blood sample DNA as a template, a concentration gradient (5×104copies/mL、1×102copies/mL) was set for repeat amplification. The CV value for the 5×104copies/mL concentration was less than 5%, and the CV value for the 1×102copies/mL concentration was less than 10%.
★Sensitivity
Using whole blood sampleDNAas a template, a concentration gradient (2.5×103copies/mL、2.5×102copies/mL、2.5×101copies/mL)was set, and the detection limit could reach25copies/mL。
★Multiplex Target
Using microbial cultures as a template, adjusted to0.5McFarland (approximately108 CFU/mL) for quadruplex target amplification, with excellent specificity.
02 Direct qPCR Premix
Product Advantages
1. Multiplex Target Amplification: Strong specificity for multiplex target amplification.
2. Excellent Repeatability:CVvalue less than10%.
3. Convenient Operation: No extraction required, saving detection time, preventing contamination, and ensuring high biosafety.
4. Products are available in lyophilized liquid, lyophilized powder, and lyophilized microsphere forms.
Product Performance Test Data
★Repeatability
Using microbial cultures as a template, adjusted to0.5McFarland (approximately108 CFU/mL) for repeatability testing,CVvalue less than10%。
★Detection Limit
Using microbial cultures as a template, adjusted to0.5McFarland (approximately108 CFU/mL) for gradient dilution, detection down to103 CFU/mL。
★Multiplex Target
Using microbial cultures as a template, adjusted to0.5McFarland (approximately108 CFU/mL) for triple target amplification, high specificity.
03 One-Step RT-qPCR Premix
Product Advantages
1. Dual Target Amplification: Strong specificity.
2. Excellent Repeatability:CVvalue less than10%.
3. Convenient Operation: No need to extractRNA，one-tube all-premixed reagent, saving detection time.
Product Performance Test Data
★Repeatability
Using whole blood samples as a template, a concentration gradient (1×100ng/T、1×10-1ng/T、1×10-2ng/T、1×10-3ng/T) was set for repeatability testing,CVless than10%。
★Dual Target
Using serum samples as a template, a concentration gradient (1×102ng/T、1×101ng/T、1×100ng/T、1×10-1ng/T、1×10-2ng/T、1×10-3ng/T) was set for dual target amplification detection of the target gene and endogenous internal standard, with excellent specificity.
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